An appropriate amount of DNA was taken and diluted to 1 ng/μL with sterile water. Genomic DNA of the samples were extracted by CTAB or SDS, and then the purity and concentrations were detected by agarose gel electrophoresis. After one week of adaptation, the mice were randomly divided into 2 groups ( n = 10): the mice on the normal diet (ND) group were fed with AIN93M standard feed (TROPHIC Animal Feed High-Tech Co., Ltd., Nantong, China) and distilled water, while the mice in the AAP group were given standard feed containing 200 mg/kg/day AAP (TROPHIC Animal Feed High-Tech Co., Ltd., Nantong, China) and distilled water. The mice were reared under standard conditions (temperature 25 ± 2 ☌, relative humidity 40 ± 10%, light–dark cycle of 12/12 h, clean bedding, and water and food ad libitum). The animal experiment protocol was approved by the Animal Ethics Committee of the Laboratory Animal Center of Northwest University (Approval Code: NWU-AWC-20200401M), and was carried out in accordance with the “Administrative Regulations on Laboratory Animals” of the National Science and Technology Commission of the People’s Republic of China. Although many recent studies have focused on using Auricularia auricula extract to prevent and treat metabolic and other disorders and as a cooking ingredient, its effect and potential mechanism on a healthy body remain unclear.Ĭ57BL/6J male mice (7-week-old, SPF-grade) were purchased from the Experimental Animal Center of Xi’an Jiaotong University (license number: SCXK 2018-001). Polysaccharides, one of the most important active components in the fruiting body of Auricularia auricula, have strong antioxidant, immune regulation, weight-reduction, and fat-decreasing properties. As the saying goes “Little fungus, big industry”, the Shaanxi Qinba Mountains, with its dense forests, sufficient sunlight, humidity and large summer-to-autumn temperature variation is also suitable for Auricularia auricula. The Chinese first cultivated Auricularia auricula 4000 years ago during the time of Emperor Shennong it was noted in Li Shizhen’s Compendium of Materia Medica during the Ming dynasty that Auricularia auricula “benefits qi, no hunger, be light and better in memory”.
The bioactivities of AAP may be related to the regulatory effects of endogenous metabolism and gut microbiota composition.Īuricularia auricula-judae, commonly known as black fungus, is a common delicious and healthy food and in China has the reputation of being the “meat in plain food”. The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
Furthermore, supplementation of AAP significantly changed the composition of the mice intestinal flora. Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline glycine, serine and threonine and glycerophospholipid, along with the sphingolipid metabolism pathway. The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component. Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
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